FastQCFastQC Report
Wed 4 Feb 2015
MK234_CTTGTA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK234_CTTGTA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8749229
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC118837613.582636824341893TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1442350.044.6826412
GCACACG1448750.044.5349411
CGTCTGA1446850.044.43016416
AGCACAC1453850.044.4266810
GTATGCC1415950.044.3003245
GTCTGAA1450700.044.29209517
CACGTCT1453450.044.2841314
AGAGCAC1462000.044.272888
TCTGAAC1450850.044.2518518
CTGAACT1450400.044.24541519
ACGTCTG1455400.044.23252515
ACACGTC1456700.044.19613613
GTAATCT1393700.044.1926937
AGTCACC1428700.044.1817728
GTCACCT1424800.044.16291429
GATCGGA1362200.044.1368031
TGTAATC1393500.044.12288736
GAGCACA1467550.044.070199
TTGTAAT1401450.044.0267335
ATCGGAA1370000.044.0226442
Produced by FastQC (version 0.11.2)