FastQCFastQC Report
Wed 4 Feb 2015
MK233_GGCTAC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK233_GGCTAC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24524433
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC4039811.6472592862799316TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT533300.043.30944412
GCTACAT487100.043.0804335
ACGGCTA500400.042.98752632
CTACATC489700.042.75052636
GGCTACA492350.042.73126234
GCACACG543000.042.63935511
CGGCTAC497250.042.62702633
TACATCT505850.041.8706737
CACGGCT515650.041.85155531
CGTCTGA550350.041.84933516
AGCACAC558700.041.55791510
CTGAACT557550.041.2249319
GTCTGAA561900.041.0651817
CGTATGC531200.040.9384444
GTATGCC531100.040.8878245
TCTGAAC564200.040.79826418
CAGTCAC552850.040.6823227
CACGTCT568550.040.60069714
ACGTCTG568500.040.57656515
TCACGGC539950.040.2671230
Produced by FastQC (version 0.11.2)