Basic Statistics
Measure | Value |
---|---|
Filename | MK232_TAGCTT_L002_R1_001.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 22937231 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC | 1146953 | 5.000398696773818 | TruSeq Adapter, Index 10 (100% over 51bp) |
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCGT | 44890 | 0.19570801724061634 | No Hit |
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAAA | 32433 | 0.1413989334632415 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CACACGT | 140265 | 0.0 | 44.22625 | 12 |
TAGCTTA | 134635 | 0.0 | 44.028915 | 34 |
GCACACG | 141095 | 0.0 | 43.982033 | 11 |
ACTAGCT | 135875 | 0.0 | 43.91176 | 32 |
CTAGCTT | 135930 | 0.0 | 43.77675 | 33 |
CGTCTGA | 141255 | 0.0 | 43.74747 | 16 |
TCACTAG | 137980 | 0.0 | 43.64948 | 30 |
GTCACTA | 138545 | 0.0 | 43.629574 | 29 |
CACTAGC | 137030 | 0.0 | 43.571983 | 31 |
CTTATCT | 136550 | 0.0 | 43.53552 | 37 |
CTGAACT | 141890 | 0.0 | 43.533043 | 19 |
TATCTCG | 138310 | 0.0 | 43.465286 | 39 |
GTCTGAA | 142285 | 0.0 | 43.463985 | 17 |
TCTGAAC | 142350 | 0.0 | 43.42685 | 18 |
AGTCACT | 140095 | 0.0 | 43.404297 | 28 |
GTATGCC | 139105 | 0.0 | 43.3545 | 45 |
AGCTTAT | 136910 | 0.0 | 43.282322 | 35 |
ACGTCTG | 143125 | 0.0 | 43.227757 | 15 |
CACGTCT | 143430 | 0.0 | 43.195435 | 14 |
TTATCTC | 139255 | 0.0 | 43.114822 | 38 |