FastQCFastQC Report
Wed 4 Feb 2015
MK232_TAGCTT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK232_TAGCTT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22937231
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC11469535.000398696773818TruSeq Adapter, Index 10 (100% over 51bp)
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCGT448900.19570801724061634No Hit
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAAA324330.1413989334632415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1402650.044.2262512
TAGCTTA1346350.044.02891534
GCACACG1410950.043.98203311
ACTAGCT1358750.043.9117632
CTAGCTT1359300.043.7767533
CGTCTGA1412550.043.7474716
TCACTAG1379800.043.6494830
GTCACTA1385450.043.62957429
CACTAGC1370300.043.57198331
CTTATCT1365500.043.5355237
CTGAACT1418900.043.53304319
TATCTCG1383100.043.46528639
GTCTGAA1422850.043.46398517
TCTGAAC1423500.043.4268518
AGTCACT1400950.043.40429728
GTATGCC1391050.043.354545
AGCTTAT1369100.043.28232235
ACGTCTG1431250.043.22775715
CACGTCT1434300.043.19543514
TTATCTC1392550.043.11482238