Basic Statistics
Measure | Value |
---|---|
Filename | MK231_GATCAG_L002_R1_001.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 9917395 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 52 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC | 484411 | 4.884458065852979 | TruSeq Adapter, Index 9 (100% over 51bp) |
GCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAA | 15153 | 0.15279213946807604 | No Hit |
GTACGATTGAGTGCAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTTC | 13545 | 0.13657820425625883 | No Hit |
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCGT | 11190 | 0.11283204914193698 | No Hit |
GCGTTCAGTTATAATCTCTCAAATGGTAGCTTCAGACCATTGATCTGTCGA | 11165 | 0.1125799668158826 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CACACGT | 60130 | 0.0 | 44.24087 | 12 |
CGTCTGA | 60635 | 0.0 | 43.77617 | 16 |
GCACACG | 61015 | 0.0 | 43.67292 | 11 |
CACGTCT | 60955 | 0.0 | 43.583263 | 14 |
GTCTGAA | 60950 | 0.0 | 43.546234 | 17 |
ACGTCTG | 61165 | 0.0 | 43.42995 | 15 |
GTATGCC | 59045 | 0.0 | 43.408016 | 45 |
TCTGAAC | 61020 | 0.0 | 43.378525 | 18 |
CTGAACT | 61170 | 0.0 | 43.239925 | 19 |
ACACGTC | 61565 | 0.0 | 43.202366 | 13 |
GATCGGA | 57615 | 0.0 | 43.19236 | 1 |
AGCACAC | 61800 | 0.0 | 43.17642 | 10 |
GAGCACA | 62155 | 0.0 | 43.031372 | 9 |
TCAGATC | 57805 | 0.0 | 42.819946 | 36 |
GATCAGA | 57930 | 0.0 | 42.786247 | 34 |
CTCGTAT | 59725 | 0.0 | 42.763363 | 42 |
ATCAGAT | 57910 | 0.0 | 42.687912 | 35 |
CGGAAGA | 59630 | 0.0 | 42.66643 | 4 |
ATCGGAA | 58580 | 0.0 | 42.663757 | 2 |
CAGATCT | 58290 | 0.0 | 42.56811 | 37 |