FastQCFastQC Report
Wed 4 Feb 2015
MK231_GATCAG_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK231_GATCAG_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9917395
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC4844114.884458065852979TruSeq Adapter, Index 9 (100% over 51bp)
GCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAA151530.15279213946807604No Hit
GTACGATTGAGTGCAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTTC135450.13657820425625883No Hit
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCGT111900.11283204914193698No Hit
GCGTTCAGTTATAATCTCTCAAATGGTAGCTTCAGACCATTGATCTGTCGA111650.1125799668158826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT601300.044.2408712
CGTCTGA606350.043.7761716
GCACACG610150.043.6729211
CACGTCT609550.043.58326314
GTCTGAA609500.043.54623417
ACGTCTG611650.043.4299515
GTATGCC590450.043.40801645
TCTGAAC610200.043.37852518
CTGAACT611700.043.23992519
ACACGTC615650.043.20236613
GATCGGA576150.043.192361
AGCACAC618000.043.1764210
GAGCACA621550.043.0313729
TCAGATC578050.042.81994636
GATCAGA579300.042.78624734
CTCGTAT597250.042.76336342
ATCAGAT579100.042.68791235
CGGAAGA596300.042.666434
ATCGGAA585800.042.6637572
CAGATCT582900.042.5681137