FastQCFastQC Report
Wed 4 Feb 2015
MK230_CAGATC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK230_CAGATC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9565950
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC926690.9687380761973458TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT125650.042.57454312
AGTCACC119100.041.60013628
GCACACG129850.041.28409211
CGTCTGA130600.040.84073616
AGCACAC135300.039.77077510
GTCACCA124050.039.3784929
GTCTGAA135700.039.2560917
CAGTCAC131750.039.1937427
CTGAACT136100.039.15785619
TCACCAG125100.038.88630330
CAGATCA123450.038.73307834
TCTGAAC138000.038.73243318
CGTATGC129950.038.39955544
GTATGCC131300.038.074945
ACCAGAT129350.037.33167332
CTCGTAT134100.037.27697442
CCAGTCA140700.036.9876826
AGAGCAC147050.036.807268
ACGTCTG145950.036.65328615
ACACGTC147400.036.41443313
Produced by FastQC (version 0.11.2)