FastQCFastQC Report
Wed 4 Feb 2015
MK228_GCCAAT_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK228_GCCAAT_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23562881
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC2162380.9177061158183502TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCC282800.042.01222230
CACGCCA288500.041.1602831
GTCACGC291500.040.933429
ACGCCAA294250.040.5646832
GTATGCC298250.040.17223745
TATCTCG302350.039.8271839
ACGTCTG307650.039.7749315
CACACGT314950.039.1246612
GCCAATA304400.039.1104934
CGTCTGA315700.038.8533416
CGCCAAT311200.038.3122833
CGTATGC314150.038.2052644
CACGTCT320800.038.1374914
GCACACG326300.037.87389411
CTCGTAT322200.037.43058442
CAGTCAC323250.037.26272227
GTCTGAA329700.037.25127417
TCGTATG327050.036.99342743
ATATCTC330350.036.40379338
CTGAACT336000.036.36659619