FastQCFastQC Report
Wed 4 Feb 2015
MK227_ACAGTG_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK227_ACAGTG_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22188291
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC3718731.675987573806383TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAC492400.041.91530629
CACACGT502600.041.9014712
GTATGCC494700.041.86130545
CACACAG493700.041.7547431
ACACAGT492700.041.7359532
CGTCTGA506650.041.34453216
GCACACG511050.041.2958511
CACAGTG500300.041.13407533
AGTCACA505000.041.0023628
ACGTCTG516000.040.66947615
CAGTCAC510050.040.5750927
TCACACA511400.040.42668530
CGTATGC512800.040.39322344
GTCTGAA520150.040.37526317
CTGAACT522300.039.9636619
TCGTATG521350.039.9543543
TCTGAAC526750.039.7199118
AGCACAC533350.039.66622510
CTCGTAT524500.039.60167742
CACGTCT532450.039.38341514
Produced by FastQC (version 0.11.2)