FastQCFastQC Report
Wed 4 Feb 2015
MK226_TGACCA_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK226_TGACCA_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23623112
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC4580321.93891473739785TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACT602950.042.45635228
CACACGT611500.042.3631612
ACTGACC598750.042.117832
GTATGCC601950.042.07336445
CGTCTGA615100.041.9028416
CTGACCA601000.041.8226433
ACGTCTG618800.041.663215
GCACACG623800.041.5990911
GTCACTG617450.041.4100529
CAGTCAC621100.041.16670627
ACCAATC607250.041.05914336
CACTGAC619850.041.02699331
GACCAAT611900.040.8394135
GTCTGAA636450.040.67391617
TCACTGA628550.040.66633230
CTGAACT633750.040.65189419
TGACCAA616500.040.62598834
CACGTCT636450.040.50073214
AGCACAC644050.040.39243710
ACACGTC640450.040.37773513
Produced by FastQC (version 0.11.2)