FastQCFastQC Report
Wed 4 Feb 2015
MK225_TTAGGC_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK225_TTAGGC_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24225594
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC5495592.26850577946613TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACTTA672300.044.3666230
ACTTAGG673400.044.31159232
CACTTAG675650.044.14222331
CTTAGGC673050.044.00815633
AGTCACT692700.043.1889528
GTATGCC690250.043.1412345
TTAGGCA686400.043.13052434
TAGGCAT692100.042.68197335
CGTATGC704600.042.2984844
ACGTCTG712600.042.23376515
GTCACTT709900.042.1444529
AGGCATC701500.042.04900436
CGTCTGA716100.041.9927916
CACACGT718550.041.88109212
CAGTCAC715550.041.85909327
CTCGTAT715450.041.696142
TCGTATG719650.041.61004643
GCACACG730100.041.2638211
CTGAACT731350.041.1169119
GTCTGAA734000.041.091317
Produced by FastQC (version 0.11.2)