FastQCFastQC Report
Wed 4 Feb 2015
MK224_CGATGT_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK224_CGATGT_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23259287
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC6279312.6997001240837695TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT791950.043.43307512
GCACACG795600.043.24720411
TATCTCG785550.043.01590739
AGTCACC790600.042.9554528
CGTCTGA799450.042.82555816
GTCACCG793600.042.7412129
TCACCGA790700.042.7276630
GTATGCC793100.042.4912445
GTCTGAA810850.042.3593917
CTGAACT807800.042.280319
AGCACAC814700.042.26662410
TCTGAAC811200.042.16641618
ATGTATC804900.041.9344536
CAGTCAC812100.041.9195627
ACCGATG808600.041.8667232
CACGTCT818450.041.77915614
CGTATGC807800.041.7368144
CACCGAT810800.041.70181731
ACGTCTG822650.041.6506215
TGTATCT812700.041.55966637
Produced by FastQC (version 0.11.2)