FastQCFastQC Report
Wed 4 Feb 2015
MK223_ATCACG_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK223_ATCACG_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7479951
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC1184561.5836467377928012TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT146650.043.02617312
AGTCACA145750.042.4468628
CGTCTGA148750.042.16166316
GCACACG150350.042.05710211
GTCTGAA150700.041.5116217
GTCACAT149150.041.49433529
CTGAACT151900.041.24346519
ACATCAC149850.041.23794632
TCTGAAC152300.041.07551618
CAGTCAC150700.041.04712727
GTATGCC151000.040.8053145
AGCACAC156850.040.34290310
CGTATGC153450.040.2427744
CTCGTAT155550.039.7552242
ACACGTC159000.039.69835313
ATCACGA155150.039.6280334
CATCACG155400.039.6069333
TCACATC156800.039.57407430
CACATCA156750.039.5638731
TCGTATG157550.039.4801443
Produced by FastQC (version 0.11.2)