FastQCFastQC Report
Wed 4 Feb 2015
lane8_Undetermined_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane8_Undetermined_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2884175
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG63210.2191614586493538No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG55130.19114651503462862No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC31890.11056888018237451No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG30990.10744840378964521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG22450.042.2322915
GCACACG22650.042.17088711
CACGTCT22700.041.7211114
ACACGTC22950.041.44716313
CACACGT23250.041.08260712
AGCACAC23400.040.90944310
CGTCTGA23100.040.8510416
AGAGCAC23500.040.8300938
CTGAACT23700.040.02715319
TCGGAAG24700.038.804753
CAGTCAC16300.038.21985627
ATCGGAA25000.038.163422
CGGAAGA25300.038.1024934
GATCGGA25150.037.9994741
CCAGTCA18150.037.30953626
AACTCCA25250.035.28130722
GAACTCC28950.032.27716421
CTCCAGT24850.032.0644324
GAGCACA30000.032.057789
TCCAGTC23650.031.20986425
Produced by FastQC (version 0.11.2)