FastQCFastQC Report
Wed 4 Feb 2015
lane7_Undetermined_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane7_Undetermined_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2891691
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG59030.20413661072362158No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG36160.12504793907786135No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA31960.11052356562302128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG49150.042.5496511
CACACGT49400.042.379512
ACACGTC49600.042.20788213
ACGTCTG49400.042.01732615
AGCACAC50050.041.87261610
CGTCTGA50050.041.4716516
CACGTCT50250.041.35114
AGAGCAC50750.041.3161168
CTGAACT50100.041.24777619
CAGTCAC34950.041.1334527
CCAGTCA37900.040.54775626
CGGAAGA53350.039.683984
TCGGAAG50000.039.6300933
GATCGGA50500.039.249191
ATCGGAA50900.038.9732062
GAGCACA54650.038.408449
CTCCAGT46550.038.09576424
AACTCCA52750.037.398822
GAACTCC54800.037.397521
ACTCCAG50750.037.06063523
Produced by FastQC (version 0.11.2)