3.6_GCCAAT_L008_R1_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	3.6_GCCAAT_L008_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15199856
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC	374118	2.461325949403731	TruSeq Adapter, Index 6 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG	88251	0.5806041846712232	No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG	74019	0.4869717186794401	No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG	44306	0.2914896035857182	No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC	43639	0.28710140411856533	No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT	41942	0.27593682466465475	No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA	35460	0.23329168381595192	No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC	34464	0.22673899015885415	No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA	27242	0.17922538213519917	No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT	26036	0.17129109644196627	No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT	23924	0.15739622796426492	No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT	23895	0.15720543668308437	No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC	23876	0.15708043549886264	No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT	23259	0.15302118651650384	No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA	22793	0.14995536799822315	No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG	22657	0.14906062267958328	No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC	21366	0.1405671211622005	No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG	20003	0.1315999309467142	No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA	19773	0.13008675871666153	No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT	19465	0.12806042373033008	No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT	19348	0.12729067959591198	No Hit
GGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATC	17904	0.11779058959505931	No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG	17470	0.11493529938704683	No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCC	17083	0.11238922263474074	No Hit
CGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTG	16145	0.10621811154000406	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCACACG	40480	43.740047	11
CACACGT	40640	43.518024	12
ACGTCTG	40785	43.269688	15
ACACGTC	40870	43.267906	13
GTATGCC	40305	43.121483	45
GTCACGC	40580	42.952477	29
AGCACAC	41325	42.927322	10
CACGTCT	41175	42.909016	14
TCACGCC	40590	42.878475	30
TATCTCG	40610	42.78762	39
CGTATGC	40605	42.785553	44
CACGCCA	40665	42.75812	31
AGTCACG	40970	42.58303	28
CGTCTGA	41535	42.504616	16
TCGTATG	40935	42.479122	43
CTCGTAT	40940	42.456184	42
ACGCCAA	41125	42.305683	32
GAGCACA	41975	42.28993	9
GCCAATA	41215	42.188637	34
CGCCAAT	41295	42.16671	33

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content