3.5_ACAGTG_L008_R1_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	3.5_ACAGTG_L008_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14486459
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC	201064	1.3879444245139547	TruSeq Adapter, Index 5 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG	84173	0.5810460651564333	No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG	75210	0.5191744925381696	No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC	44382	0.30636886488271564	No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG	39372	0.27178484403952685	No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT	33904	0.23403925003342776	No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA	30900	0.21330264352385908	No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC	27887	0.19250391003073974	No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA	26600	0.18361975138299844	No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT	26482	0.1828051975986678	No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC	25606	0.17675817119973902	No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT	24693	0.17045573386843532	No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA	24342	0.16803278150996045	No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG	23956	0.16536822421545527	No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT	23623	0.16306952582408166	No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT	22303	0.15395756823665466	No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG	21290	0.14696483108812167	No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC	20851	0.14393441489048497	No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG	20783	0.14346501101476905	No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT	19188	0.1324547289299614	No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA	18261	0.12605564962424565	No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT	17861	0.12329445035532838	No Hit
GGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATC	17079	0.11789630578459512	No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCC	16174	0.1116490924386698	No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATGT	15631	0.1079007644311146	No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC	15559	0.10740374856270948	No Hit
GCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGG	15112	0.10431810837969445	No Hit
GGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC	14736	0.10172258106691222	No Hit
GGGGAAGTTGGTGCCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTG	14676	0.10130840117657462	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCACACG	22195	42.57891	11
GTATGCC	21780	42.57741	45
CGTATGC	21865	42.380135	44
ACGTCTG	22320	42.13888	15
CACACGT	22520	41.96443	12
CTCGTAT	22190	41.726402	42
ACACGTC	22630	41.70066	13
AGCACAC	22780	41.495342	10
TCGTATG	22355	41.471195	43
CACGTCT	22890	41.187687	14
CACAGTG	22605	40.824577	33
GTCACAC	22790	40.69899	29
CGTCTGA	23180	40.633717	16
ACACAGT	22795	40.53142	32
CAGTCAC	23045	40.295097	27
TCTCGTA	23055	40.168827	41
GAGCACA	23625	40.09742	9
CACACAG	23145	40.07516	31
AGAGCAC	23765	39.85174	8
CTGAACT	23740	39.676033	19

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content