FastQCFastQC Report
Wed 4 Feb 2015
2.9_GATCAG_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2.9_GATCAG_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15708724
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG638940.4067421389541251No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG441730.28120043359346053No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC391270.24907815555229054TruSeq Adapter, Index 9 (100% over 51bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA382460.24346980696840814No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC289640.1843816213207387No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG259040.1649019996786499No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC252690.1608596598934452No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT231910.14763134166721625No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT205350.1307235393530372No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA204620.1302588294249743No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG193990.12349188896564738No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG184380.1173742692277234No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT167010.10631671929559651No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA163140.10385312008792057No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA162530.1034648008329639No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT159300.10140861854852118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG56050.036.84415411
CGTATGC52650.036.37996344
GTATGCC53850.035.65331345
ACGTCTG61400.033.7433815
AGCACAC63100.032.94124210
GTCACGA58000.032.89732729
AGTCACG57550.032.8816128
ACACGTC62950.032.87679313
CACACGT62950.032.62695312
CTCGTAT60350.032.03500442
TCGTATG60150.031.99327943
CACGTCT66800.031.08297514
CGTCTGA69350.029.94024716
GAGCACA69950.029.586679
AGAGCAC72050.028.8493028
CAGTCAC67150.028.44899227
TCTCGTA68300.028.3711941
TCACGAT68900.027.69710430
CGATCAG69200.027.5742233
CTGAACT75250.027.4736319
Produced by FastQC (version 0.11.2)