FastQCFastQC Report
Wed 4 Feb 2015
2.8_ACTTGA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2.8_ACTTGA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18205111
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG794020.4361522431805003No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC580880.3190752311260283TruSeq Adapter, Index 8 (100% over 51bp)
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG448260.24622755664604296No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA420970.23123726078901688No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG407010.2235690845279658No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC364310.20011413278391987No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC318710.17506622178793638No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA268030.14722788561959332No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT250190.13742843973870855No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT208180.11435250243736499No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT204950.11257827540848281No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG196490.10793122876317535No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG193810.10645911469586754No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT189150.10389939396689203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG79550.037.29959511
GTATGCC77550.037.2957645
CGTATGC77650.037.21761344
ACGTCTG84800.035.01640715
CTCGTAT83500.034.68892342
AGCACAC86300.034.5645610
ACACGTC86400.034.31588413
TCGTATG85300.034.1433943
CACACGT87400.034.00093512
GTCACAC87950.032.79816429
CACGTCT91250.032.56592614
CGTCTGA92000.032.44715516
GAGCACA95950.031.1117369
AGAGCAC98000.030.5302968
TCTCGTA95650.030.35196141
CAGTCAC96500.030.03227627
CTGAACT102250.029.0197819
CCAGTCA101200.028.59302726
GCTGACT72450.027.9063221
TCCAGTC107150.027.53025625
Produced by FastQC (version 0.11.2)