FastQCFastQC Report
Wed 4 Feb 2015
2.7_CAGATC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2.7_CAGATC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18783879
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC1872580.9969080401337763TruSeq Adapter, Index 7 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG849330.4521590029407664No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG546810.2911060063791936No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA491910.26187881640421556No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC381200.2029399784783537No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG368720.1962959833802166No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT329080.1751927810011979No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC308170.1640608949834057No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA292380.15565475054433647No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT225920.12027334716114813No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG223830.119160690930771No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT220540.11740918901788071No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT219960.11710041360466601No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG214700.11430014002964989No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT209390.11147324788452907No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC207980.11072260420757608No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT205850.10958865312111518No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA202900.10801815748493694No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC192630.1025507031854283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC228050.041.5704345
CGTATGC228500.041.47782544
GCACACG233950.041.4247911
ACGTCTG237250.040.8002115
CTCGTAT234800.040.42071542
TCGTATG235650.040.35271543
AGCACAC240650.040.30875410
CACACGT241900.040.04477312
ACACGTC243700.039.74804313
CACGTCT244750.039.57752214
CATCTCG239950.039.4054539
CGTCTGA247400.039.1082316
TCTCGTA244350.038.80399341
GAGCACA250950.038.7350049
AGAGCAC252750.038.5576638
CAGTCAC249250.038.2407827
AGTCACC248900.038.0682428
CTGAACT257550.037.57691619
CCAGTCA255300.037.45795426
TCCAGTC259700.037.0312625
Produced by FastQC (version 0.11.2)