2.6_GCCAAT_L007_R1_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	2.6_GCCAAT_L007_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16694131
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC	379296	2.2720320093331003	TruSeq Adapter, Index 6 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG	84283	0.5048660514284931	No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG	51224	0.30683837331814395	No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA	46091	0.27609104061780754	No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC	37510	0.2246897427604947	No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG	33593	0.20122640705287384	No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT	29843	0.17876342290593022	No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC	28018	0.16783143728775102	No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA	27603	0.1653455337088226	No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG	22797	0.13655697322609964	No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT	21751	0.13029129818137883	No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG	20836	0.12481033004952459	No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT	20803	0.12461265578903148	No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA	20798	0.12458270514350224	No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA	19328	0.11577721535790034	No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT	19258	0.11535790632049071	No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT	18934	0.11341710449019478	No Hit
CCGGCGCCTACAAGACCGACATCAAGCTGGACATCACCTCCCACAACGAGG	18861	0.11297982506546762	No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC	18521	0.11094318116947806	No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT	18478	0.11068560561792644	No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC	17413	0.10430611812019445	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTATGCC	44615	43.00402	45
GCACACG	45840	42.970673	11
CGTATGC	44980	42.64954	44
TCACGCC	45105	42.574757	30
GTCACGC	45310	42.509342	29
ACGTCTG	46230	42.505093	15
AGCACAC	46450	42.444984	10
AGTCACG	45475	42.369946	28
CTCGTAT	45295	42.328243	42
CACGCCA	45385	42.288586	31
CACACGT	46600	42.260212	12
TATCTCG	45570	42.192993	39
ACACGTC	46700	42.1592	13
TCGTATG	45635	42.071285	43
CGTCTGA	46830	41.951523	16
GCCAATA	45700	41.933975	34
CACGTCT	46915	41.90366	14
ACGCCAA	45890	41.900414	32
GAGCACA	47200	41.799137	9
CGCCAAT	46035	41.667793	33

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content