FastQCFastQC Report
Wed 4 Feb 2015
2.5_ACAGTG_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2.5_ACAGTG_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17551455
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1638910.9337744363643926TruSeq Adapter, Index 5 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG783050.44614534806373607No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG498980.28429551851968965No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA466800.2659608562367052No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC355920.2027866065804801No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG348820.19874135791021313No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC283910.16175866901063188No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA278750.15881874180801533No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT270980.15439175840407535No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG231150.13169848311721166No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT224030.1276418393802679No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG209830.11955134203973403No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT207500.11822381677188587No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA205610.11714698297092749No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT197720.11265162916692661No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT191840.10930147956394497No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT191250.10896532509697915No Hit
CCGGCGCCTACAAGACCGACATCAAGCTGGACATCACCTCCCACAACGAGG182680.10408253902596679No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC179240.10212258755755578No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC176800.10073238942298517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC199000.041.58817344
GTATGCC199100.041.56847445
GCACACG204300.041.53470611
ACGTCTG206150.040.94301615
AGCACAC210950.040.27833610
ACACGTC211150.040.1226513
CTCGTAT206500.040.09699242
TCGTATG206950.040.0555943
CACACGT211600.040.04863712
CACGTCT212500.039.8359914
CACAGTG208650.039.4599933
ACACAGT209050.039.4255232
CGTCTGA214850.039.3166216
GAGCACA217900.039.107219
CACACAG211700.039.07106431
GTCACAC212850.038.98493629
AGAGCAC221250.038.515628
TCTCGTA215750.038.35669741
CAGTCAC219750.037.7623427
CTGAACT224000.037.7621119
Produced by FastQC (version 0.11.2)