FastQCFastQC Report
Wed 4 Feb 2015
2.4_TGACCA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2.4_TGACCA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21305083
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC19722879.257354219178588TruSeq Adapter, Index 4 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG860740.404006874791335No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG541590.2542069420710541No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA508900.23886318584161345No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC414450.19453104219307663No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG359320.16865458820320015No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC300490.1410414594489024No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA295160.13853970904501992No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT274810.12898799784070308No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG249390.1170565728375712No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT230050.10797892690678557No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA226750.10643000076554501No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG221320.10388131320586734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG2274800.044.11355611
AGCACAC2279500.044.01460310
GTATGCC2227600.043.93538345
ACGTCTG2279600.043.92936315
CACACGT2282050.043.92510612
ACACGTC2284100.043.8772913
GAGCACA2291550.043.8538329
AGAGCAC2291950.043.852388
CACGTCT2285050.043.84132814
CGTCTGA2287800.043.73977316
TCGGAAG2169650.043.7386633
CGTATGC2243800.043.62877744
ACTGACC2253000.043.58762732
CTCGTAT2249800.043.55109442
CTGAACT2297850.043.52042419
CGGAAGA2319350.043.494314
TCGTATG2255550.043.4769343
AGTCACT2274950.043.4673228
CACTGAC2273150.043.4324231
GTCTGAA2306350.043.39480217
Produced by FastQC (version 0.11.2)