2.2_CGATGT_L007_R1_001.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	2.2_CGATGT_L007_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14429662
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC	429259	2.974837525646824	TruSeq Adapter, Index 2 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG	67677	0.4690130648936891	No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG	43911	0.30431066230102966	No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA	39611	0.27451093449035746	No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC	33871	0.23473176294773918	No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG	32879	0.22785703504350968	No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC	28789	0.1995126427770796	No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT	19746	0.1368431221743101	No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT	19503	0.1351590910445442	No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG	19167	0.1328305541737568	No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA	18660	0.1293169583598008	No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG	17826	0.12353719719838205	No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA	17807	0.12340552398247444	No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA	17785	0.12325306025879192	No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT	17338	0.12015527460033368	No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT	16648	0.11537345781210953	No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT	16235	0.11251129790843332	No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT	16100	0.1115757250585634	No Hit
CCGGCGCCTACAAGACCGACATCAAGCTGGACATCACCTCCCACAACGAGG	15430	0.10693251165550517	No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC	15255	0.1057197320353034	No Hit
GCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCA	15015	0.10405649141331239	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCACACG	52145	43.106636	11
GTATGCC	49885	43.04375	45
CGTATGC	50105	42.880962	44
ACGTCTG	52475	42.809532	15
CACACGT	52560	42.740597	12
AGCACAC	52550	42.74017	10
TATCTCG	50310	42.717495	39
CTCGTAT	50495	42.6055	42
ACACGTC	52760	42.591076	13
CACGTCT	52795	42.575626	14
TCGTATG	50595	42.541	43
CGATGTA	50720	42.393482	34
CGTCTGA	53025	42.340332	16
GAGCACA	53245	42.224537	9
CCGATGT	51135	42.106632	33
CTGAACT	53320	41.9507	19
TCTCGTA	51360	41.882977	41
CAGTCAC	51725	41.814415	27
AGAGCAC	53805	41.79012	8
ACCGATG	51830	41.72175	32

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content