FastQCFastQC Report
Wed 4 Feb 2015
2.1_ATCACG_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2.1_ATCACG_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16331957
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG770070.471511160603717No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC605360.3706598051905231TruSeq Adapter, Index 1 (100% over 51bp)
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG482520.2954453039522453No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA447780.27417412377463396No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC387930.23752817864999276No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG325950.1995780419945999No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC281660.1724594303058721No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA236980.14510202298475314No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG225840.13828104004927272No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT225100.13782794064422288No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT213970.13101308067367554No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA207380.12697804678275848No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG205560.12586366716493314No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA200670.12286953731264416No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT185210.11340343352606182No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT181770.11129713358907324No Hit
CCGGCGCCTACAAGACCGACATCAAGCTGGACATCACCTCCCACAACGAGG178380.10922144847675022No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT176080.10781316654213577No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT166800.10213105508421311No Hit
GCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCA166390.10188001352195576No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC163980.10040437897307715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC86100.035.658845
CGTATGC86450.035.43490644
TCGTATG93300.032.95342643
CTCGTAT94450.032.45458642
TCTCGTA101900.030.03707741
TCACGAT103250.029.61718835
GTCACAT104700.029.37805429
CACGATC106500.028.64973336
CAGTCAC108000.028.64776627
ACGTCTG129250.028.50883315
ATCACGA108400.028.31371934
GCACACG132350.027.70569611
CTGAACT132900.027.4555419
ACGATCT112150.027.22611837
CGTCTGA136050.027.15013916
CACGTCT135600.027.09084914
CCAGTCA115650.026.9084326
ACACGTC136450.026.88911813
CACACGT137300.026.70683912
AGTCACA115500.026.67054628
Produced by FastQC (version 0.11.2)