FastQCFastQC Report
Wed 4 Feb 2015
2.12_CTTGTA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2.12_CTTGTA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18605030
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG896150.48167081697798925No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG591260.3177957788834525No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC502310.2699861274074807TruSeq Adapter, Index 12 (100% over 51bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA495520.2663365767214565No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC392640.2110397027040537No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG350740.188518911283669No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC289190.15543645992508476No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT288240.1549258453224746No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA276480.14860497403121628No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG248860.13375952632164528No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT247200.13286729448971596No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG228200.12265500243751287No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA219300.11787134984463879No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA218100.11722636297818387No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT216830.11654375187785239No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT198920.10691732289601252No Hit
CCGGCGCCTACAAGACCGACATCAAGCTGGACATCACCTCCCACAACGAGG197090.10593371792466877No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT193630.10407400579305703No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC193510.10400950710641155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC68400.036.6246945
GCACACG72000.036.61865211
CGTATGC69200.036.07001544
ACGTCTG78100.033.9014815
AGCACAC80000.033.2659310
ACACGTC79750.033.17186413
CACACGT80100.032.85947412
CTCGTAT76350.032.8382142
TCGTATG76350.032.75092343
CACGTCT83750.031.64125614
CGTCTGA86600.030.6261716
GAGCACA88650.030.1722729
AGAGCAC89050.029.8350458
AGTCACC88050.029.23500328
CAGTCAC87150.029.2272427
TCTCGTA86100.029.06630741
CTGAACT94600.028.20411519
CCAGTCA95500.026.90738926
TGTAATC95550.026.3750136
TCCAGTC100800.026.31855625
Produced by FastQC (version 0.11.2)