FastQCFastQC Report
Wed 4 Feb 2015
2.11_GGCTAC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2.11_GGCTAC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18019572
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG886740.492098258493598No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG543140.3014167040149455No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA533860.29626674817803667No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC404880.22468902147065425TruSeq Adapter, Index 11 (100% over 51bp)
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC382890.21248562396487553No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG350360.19443303092881453No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT285340.1583500429421964No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA280380.1555974803397106No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC253060.1404361879405349No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT251780.1397258492044095No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG236050.1309964520799939No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG230040.12766118973303028No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA219170.12162885999734067No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA213290.11836574142826478No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT210180.11663984028033518No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT208080.1154744407913795No Hit
CCGGCGCCTACAAGACCGACATCAAGCTGGACATCACCTCCCACAACGAGG194510.10794374028417544No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC183070.10159508783005503No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC181170.10054067876861891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG57450.035.5938611
GTATGCC56250.034.41295245
CGTATGC57150.033.752344
ACGTCTG62600.032.5574515
AGCACAC64750.031.68510410
CGGCTAC60900.031.29524233
AGTCACG62850.031.03970328
ACGGCTA62650.030.85043732
CACACGT66600.030.80504812
CTCGTAT63550.030.67031142
ACACGTC67400.030.47244813
CACGGCT64350.030.00154931
TCGTATG65300.029.6773543
GGCTACA64550.029.49078234
CACGTCT70050.029.38389814
GCTACAT66050.028.82112535
CGTCTGA72050.028.6306916
GAGCACA73800.028.1958689
AGAGCAC74350.027.8965998
CATCTCG70550.027.23846839
Produced by FastQC (version 0.11.2)