FastQCFastQC Report
Wed 4 Feb 2015
lane1_Undetermined_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane1_Undetermined_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4552280
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTG35050.013.5815133
TTGTGTT35350.013.2360771
CTACCCG2602.8321665E-912.92965316
TGTGTTT36850.012.6178762
AGGCTAC3351.05501385E-810.69701513
TGTTTGA47800.010.070971
GCTACCC3653.9986844E-89.817715
GACGGGC4051.9817344E-78.8477589
CTAAGCG4152.4614928E-78.72318841
ACGGGCG4407.003291E-78.1439610
TACTAAG4303.8209364E-67.89113439
ACTAAGC4852.6100279E-67.463342740
CAGGGAC7701.2369128E-107.28900631
TGTGTAC7701.2914825E-107.27650818
TTTGAGG9600.07.23192646
GGGCAGG7657.3123374E-107.0806828
CAAAGGG8308.185452E-117.06696624
GGGACGT7801.1841621E-96.925578633
GGCAGGG7851.2769306E-96.90142729
TTTGTGT27400.06.88624052
Produced by FastQC (version 0.11.2)