FastQCFastQC Report
Wed 4 Feb 2015
foxfnk412hpf2_AAGACG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefoxfnk412hpf2_AAGACG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32736996
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTG381400.012.5692563
TTGTGTT401150.011.8871471
TGTGTTT406450.011.7564472
TGTTTGA535650.09.9152881
TTTGTGT249600.09.4098912
GTTTGAG297550.08.25641252
TGACGGG68350.08.0308248
GACGGGC55500.07.94459729
ACGGGCG54900.07.293843310
CTACCCG37000.07.235268613
CTTTGTG342000.07.15586141
TAACTTT203500.07.13598251
GAGGCTA36100.07.1040579
AACTTTG212150.06.7549962
TTTGAGG127150.06.70547633
TTGACGG86500.06.6578197
GCTACCC43150.06.516794712
AGGCTAC41000.06.419641510
CGGTGTG74600.06.392996315
TGTGTAC89550.06.33058318
Produced by FastQC (version 0.11.2)