FastQCFastQC Report
Wed 4 Feb 2015
foxfnk412hpf1_CCTTCA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefoxfnk412hpf1_CCTTCA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35792511
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTG402450.013.2421993
TTGTGTT406600.013.13452151
TGTGTTT427550.012.3656192
TGTTTGA524900.010.1744684
GACGGGC46450.08.6691789
ACGGGCG47850.08.03942110
GTTTGAG305750.07.77707155
TTTGAGG124550.07.27893266
TGACGGG69000.07.00970948
TTTGTGT246700.06.8486612
TAACTTT256300.06.76408431
AGGGACG67800.06.60479932
GCGGTGT63150.06.590394514
TTGACGG81000.06.5266617
CGGTGTG64500.06.417542515
AACTTTG255950.06.3989862
CAGGGAC73200.06.240586831
GGGACGT71500.06.23140133
GGGCAGG76150.06.086972728
GGCAGGG78100.05.84867629