FastQCFastQC Report
Wed 4 Feb 2015
foxflaz12hpf2_TTCAGC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefoxflaz12hpf2_TTCAGC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32347245
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTG346800.012.9605953
TTGTGTT351900.012.8984361
TGTGTTT362200.012.4041312
TGTTTGA484900.010.5771181
TTTGTGT219750.08.6509652
GACGGGC51250.08.559539
GAGGCTA32750.08.38022512
CTACCCG33150.08.27931216
ACGGGCG49400.08.24253610
AGGCTAC34700.08.10399413
TGACGGG63550.08.0710218
GTTTGAG307800.07.71117542
GCTACCC38450.07.372115615
TAACTTT216150.07.21844771
AACTTTG206900.07.17662332
TTGACGG79400.07.1681747
TTTGAGG117600.07.0778763
TTTGAGT83400.06.87832453
CTAAGCG42350.06.74858341
CGGTGTG65800.06.530184315
Produced by FastQC (version 0.11.2)