FastQCFastQC Report
Wed 4 Feb 2015
foxflaz12hpf1_AAGGGA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefoxflaz12hpf1_AAGGGA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30296819
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTG319250.013.2332913
TTGTGTT319200.013.2321661
TGTGTTT331600.012.6460852
TGTTTGA429500.010.1904671
CTACCCG22600.09.65547516
TTTGTGT192500.09.3143442
GAGGCTA23300.08.59283612
AGGCTAC24550.08.15552213
TTTGAGG104700.07.8639326
GCTACCC28300.07.551748815
GACGGGC41800.07.42695869
TGACGGG53350.07.37924348
ACGGGCG42150.07.09842910
GTTTGAG311700.06.8205552
TTTGAGT78450.06.76740653
TAACTTT205650.06.4592831
CTAAGCG32950.06.419858541
GCGGTGT53050.06.403271714
CTTTGTG293650.06.38669251
AACTTTG205600.06.31360532
Produced by FastQC (version 0.11.2)