FastQCFastQC Report
Wed 4 Feb 2015
foxfcamras12hpf2_CCTCGG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefoxfcamras12hpf2_CCTCGG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32036431
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTG346150.013.563483
TTGTGTT352600.013.287311
TGTGTTT351600.013.2771842
TGTTTGA477900.010.0956081
GACGGGC51300.09.9547029
ACGGGCG52300.09.50627510
TGACGGG68750.08.7041988
TTTGTGT219300.08.114742
TTGACGG80800.07.90723667
GCGGTGT67500.07.86549414
GAGGCTA28450.07.749247612
CTACCCG27300.07.746061316
CGGTGTG68300.07.707453315
TAACTTT205700.07.70523171
AACTTTG196050.07.59675072
GTTTGAG302300.07.5314592
TTTGAGG114200.07.52508976
TGTGTAC83450.07.386521318
AGGGACG82250.07.304971732
GCTACCC31200.07.13832515