FastQCFastQC Report
Thu 22 Jan 2015
lane4_Undetermined_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane4_Undetermined_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5541311
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC179130.323262852418859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA50700.042.533411
ACACGTC44250.042.24230213
ATCGGAA51250.041.7248422
GCACACG46000.041.4665411
CGTCTGA43650.041.3322616
ACGTCTG44050.041.31068415
AATTCGG24650.041.06900423
CGGAAGA51300.040.8894
CGGTCTC24800.040.8150840
GAATTCG24850.040.74214622
TCGGAAG52150.040.615473
TTCGGTA25050.040.4077425
CACGTCT46050.040.34690514
ATTCGGT25200.040.2619324
TCGGGGA25650.039.4660917
CACACGT47850.039.2992412
CCAGGCG26500.038.36651635
CTCGGGG22250.037.40853516
CCTCGGG22250.037.30406615
TCCTCGG22600.037.12446214
Produced by FastQC (version 0.11.2)