FastQCFastQC Report
Thu 22 Jan 2015
lane3_Undetermined_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane3_Undetermined_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4967305
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC109020.2194751479927244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA58400.042.796141
ATCGGAA58400.042.6804772
ACACGTC52600.042.0456613
CGTCTGA51350.041.97508216
CGGAAGA58500.041.7607574
TCGGAAG59250.041.6881643
ACGTCTG52500.041.35436615
AATTCGG16000.041.06098623
CACGTCT53550.040.9636414
GCACACG55250.040.5575511
CACACGT55700.039.98753412
TTCGGTA16500.039.95266725
GAATTCG16700.039.34105722
ATTCGGT16900.038.87430624
CGGTCTC17300.037.71347440
TCGGGGA17250.037.69426317
CCAGGCG17400.037.49672735
TATTCGT14400.036.9014971
CAGGCGG18650.034.98354336
AGCACAC65550.034.8367810
Produced by FastQC (version 0.11.2)