FastQCFastQC Report
Thu 22 Jan 2015
lane2_Undetermined_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane2_Undetermined_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4506497
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC369140.8191284716266315No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA93540.20756698606478602No Hit
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC85070.18877189977048692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGT39050.043.141411
GATCGGA43600.043.1334081
ATCGGAA43350.043.018482
AATTCGG49050.042.66076323
TTCGGTA49000.042.6100925
GAATTCG49050.042.56901622
TCGGAAG43650.042.5164263
ACACGTC38600.042.1971313
TCGGGGA49650.042.05039217
ATTCGGT49800.042.01734524
CGGTCTC50150.041.76479740
CGGAAGA44400.041.2900664
CGTCTGA38450.041.13302216
ACGTCTG39000.040.89905515
CAGGCGG51350.040.8326136
CCAGGCG51400.040.7053535
TCGTTGG41500.040.7021454
AGCCGAT9750.040.6128818
CTCGGGG41550.040.5006716
TCCTCGG41750.040.19888714