FastQCFastQC Report
Thu 22 Jan 2015
bgm5_CAGATC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebgm5_CAGATC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36322984
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC5125051.4109661254703083TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA568500.043.6449471
ATCGGAA572400.043.3318442
CGTCTGA568200.043.04154216
ACACGTC570450.043.01746713
CTCGTAT562050.042.8517842
ACGTCTG572700.042.7581115
TCTCGTA563650.042.75408641
TCGGAAG579900.042.7326053
CGGAAGA580400.042.613824
TCGTATG566850.042.54051243
CGTATGC567150.042.4466144
ATCTCGT571450.042.20988540
CATCTCG572050.042.09482239
CACGTCT584500.041.95263714
GCACACG592650.041.48580611
CACACGT599500.041.0380512
GTATGCC591100.040.70012345
GATCATC609450.039.5595936
AGTCACC632950.038.02329328
GAACTCC642250.037.91105321
Produced by FastQC (version 0.11.2)