FastQCFastQC Report
Thu 22 Jan 2015
bgm4_GCCAAT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebgm4_GCCAAT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29183423
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC8510582.9162377559342505TruSeq Adapter, Index 6 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA3405291.1668576369536912No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC2601110.8912970901322987No Hit
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC586180.20086060500853517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA944450.044.2117081
ATCGGAA946500.044.1849142
TCGGAAG952550.043.7814063
CGTCTGA941150.043.63537216
ACGTCTG943250.043.59062615
ACACGTC945800.043.5777713
CGGAAGA955150.043.5276154
TATTCGT275200.043.3300361
TAAGCCG368350.043.27553616
CTCGTAT935350.043.1794442
CGCCAAT934500.043.16575233
TCTCGTA936250.043.14514541
AGCCGAT369500.043.11158818
GTCACGC939350.043.00994529
TATCTCG940550.043.0005239
TCGTATG940450.042.9907443
ACGCCAA939900.042.9871732
AGTCACG942400.042.9304328
CCGATGA371050.042.9277120
CGTATGC941750.042.89555744