FastQCFastQC Report
Thu 22 Jan 2015
bgm3_ACAGTG_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebgm3_ACAGTG_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29254740
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC2156630.7371899391346496TruSeq Adapter, Index 5 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA544500.18612368457214112No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC433220.1480854042797851No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA246650.043.3456541
ATCGGAA248950.042.9090272
CGTCTGA249900.042.17054716
CTCGTAT246700.042.0426142
GATCTCG246800.041.9891139
TCTCGTA247250.041.9672941
TCGTATG248100.041.88698243
ACACGTC252200.041.85725813
CGTATGC249000.041.7355844
TCGGAAG256600.041.5596163
ACGTCTG255850.041.2425315
ATCTCGT253900.040.82379540
CGGAAGA261900.040.666814
CACGTCT264000.040.04602414
GCACACG269300.039.26624711
CACACGT271650.038.9596912
AGCCGAT64800.038.81883618
TAAGCCG66000.038.21380616
AATTCGG62950.038.10458423
GTATGCC273850.038.03052545
Produced by FastQC (version 0.11.2)