FastQCFastQC Report
Thu 22 Jan 2015
bgm2_TGACCA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebgm2_TGACCA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28168105
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC2620230.9302116702561283TruSeq Adapter, Index 4 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA314270.1115694506250953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA300600.043.3463021
ATCGGAA302850.043.0391272
TCGGAAG308350.042.132753
ACACGTC302950.042.1310213
CGTCTGA302250.042.1022716
TCTCGTA298500.041.7415641
ACGTCTG305500.041.72779515
CTCGTAT298750.041.6840442
CGGAAGA311400.041.654734
AATCTCG299900.041.58421739
TCGTATG302650.041.3104443
CGTATGC302600.041.28752544
ATCTCGT304100.040.965540
CACGTCT314600.040.5851614
GCACACG319400.040.2288311
CACACGT321100.039.9527812
GTATGCC323900.038.5515845
ACCAATC338550.036.8434736
GACCAAT342850.036.51262335
ACTGACC348650.036.17624732
Produced by FastQC (version 0.11.2)