FastQCFastQC Report
Thu 22 Jan 2015
bgm1_CGATGT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebgm1_CGATGT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33393557
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC2609250.7813633031066441TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA294600.043.221571
ATCGGAA297400.042.807072
CGATGTA291500.042.41973534
CACCGAT292750.042.36157631
ACCGATG292650.042.35298532
TCTCGTA292700.042.28425641
CTCGTAT293300.042.19775842
CGTCTGA298750.042.02315516
TCGTATG295900.041.94862743
CCGATGT295350.041.8972533
ACACGTC300950.041.86522713
TATCTCG295900.041.85738839
CGTATGC296500.041.78786544
GTCACCG297700.041.6496529
TCGGAAG306300.041.5338553
ACGTCTG303250.041.47350715
ATCTCGT301250.041.17377540
CGGAAGA308900.041.07464
TCACCGA302350.041.0165430
CACGTCT312650.040.29854614
Produced by FastQC (version 0.11.2)