FastQCFastQC Report
Thu 22 Jan 2015
SV75_CGATGT_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV75_CGATGT_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61647926
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC5895720.9563533410677919TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGAT642150.042.88076831
ACCGATG643200.042.8282632
CGTCTGA653850.042.7303116
CGATGTA642750.042.72522434
TCTCGTA645700.042.6275941
CTCGTAT645950.042.56929842
ACACGTC660850.042.4231713
GTCACCG651100.042.38117229
GATCGGA669600.042.3792651
CCGATGT649250.042.3494633
ACGTCTG661600.042.2799315
TATCTCG651050.042.25656539
ATCTCGT659850.041.72029540
TCACCGA660850.041.64694630
ATCGGAA681750.041.604182
TCGTATG666500.041.3107843
CGTATGC665950.041.30435644
CACGTCT681850.041.10009814
CGGAAGA688750.041.034214
GCACACG686300.040.94506511