FastQCFastQC Report
Thu 22 Jan 2015
SV74_CTTGTA_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV74_CTTGTA_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66865529
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC11189771.6734736369168632TruSeq Adapter, Index 12 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA779240.11653837360652602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA1232700.043.21811316
ACACGTC1238150.043.16549713
ACGTCTG1240850.042.9973615
GATCGGA1266550.042.97171
TCTCGTA1225600.042.753941
CTCGTAT1226550.042.72628842
ATCGGAA1275850.042.642592
AATCTCG1237100.042.38919439
CGGAAGA1278300.042.3530464
ATCTCGT1243150.042.1557540
GCACACG1271550.042.13605511
CACGTCT1268850.042.13528414
TCGGAAG1291400.042.0506863
CGTATGC1247350.042.0498844
TCGTATG1250550.041.99805543
CACACGT1282150.041.74558612
GTATGCC1280550.040.9965845
GTAATCT1333300.039.33073837
AGTCACC1354750.038.97372428
CACCTTG1361800.038.70917531