Basic Statistics
Measure | Value |
---|---|
Filename | SV74_CTTGTA_L004_R1_001.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 66865529 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 39 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC | 1118977 | 1.6734736369168632 | TruSeq Adapter, Index 12 (100% over 51bp) |
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA | 77924 | 0.11653837360652602 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTCTGA | 123270 | 0.0 | 43.218113 | 16 |
ACACGTC | 123815 | 0.0 | 43.165497 | 13 |
ACGTCTG | 124085 | 0.0 | 42.99736 | 15 |
GATCGGA | 126655 | 0.0 | 42.9717 | 1 |
TCTCGTA | 122560 | 0.0 | 42.7539 | 41 |
CTCGTAT | 122655 | 0.0 | 42.726288 | 42 |
ATCGGAA | 127585 | 0.0 | 42.64259 | 2 |
AATCTCG | 123710 | 0.0 | 42.389194 | 39 |
CGGAAGA | 127830 | 0.0 | 42.353046 | 4 |
ATCTCGT | 124315 | 0.0 | 42.15575 | 40 |
GCACACG | 127155 | 0.0 | 42.136055 | 11 |
CACGTCT | 126885 | 0.0 | 42.135284 | 14 |
TCGGAAG | 129140 | 0.0 | 42.050686 | 3 |
CGTATGC | 124735 | 0.0 | 42.04988 | 44 |
TCGTATG | 125055 | 0.0 | 41.998055 | 43 |
CACACGT | 128215 | 0.0 | 41.745586 | 12 |
GTATGCC | 128055 | 0.0 | 40.99658 | 45 |
GTAATCT | 133330 | 0.0 | 39.330738 | 37 |
AGTCACC | 135475 | 0.0 | 38.973724 | 28 |
CACCTTG | 136180 | 0.0 | 38.709175 | 31 |