FastQCFastQC Report
Thu 22 Jan 2015
SV73_CAGATC_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV73_CAGATC_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63390792
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC11059991.7447313168133316TruSeq Adapter, Index 7 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA1107090.17464523869649712No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC858490.13542818647856616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1223200.043.7205161
CGTCTGA1209150.043.64513416
ACACGTC1214950.043.49322513
ACGTCTG1216900.043.3811115
CTCGTAT1201000.043.1985142
TCTCGTA1203000.043.132341
ATCGGAA1239150.043.1232452
CGGAAGA1241650.042.909464
GCACACG1237900.042.74686411
ATCTCGT1217350.042.6626740
TCGGAAG1255600.042.5331763
CACGTCT1244150.042.48329514
CATCTCG1222200.042.42158539
CGTATGC1227950.042.232144
CACACGT1253650.042.2134112
TCGTATG1231250.042.19748743
GTATGCC1250900.041.48965545
GATCATC1275400.040.6732536
CCAGATC1297650.039.8943633
GAACTCC1335950.039.37911621