FastQCFastQC Report
Thu 22 Jan 2015
SV72_GCCAAT_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV72_GCCAAT_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59371772
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC14255012.4009743216018546TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1571750.043.742571
CGTCTGA1550900.043.7093516
ACACGTC1552800.043.68222413
ACGTCTG1556600.043.5744715
CGCCAAT1536600.043.42075333
GTCACGC1542200.043.30247529
ATCGGAA1589950.043.2814942
TCTCGTA1540250.043.2711241
CTCGTAT1540100.043.26510242
ACGCCAA1549150.043.1009532
TATCTCG1551450.043.07620239
GCACACG1579250.043.05019411
CGGAAGA1594050.043.04874
ATCTCGT1551200.042.97436540
CACGTCT1580250.042.9419414
CACGCCA1554100.042.91154531
AGTCACG1558400.042.88698628
TCACGCC1554500.042.8816930
TCGGAAG1605700.042.830323
CACACGT1587250.042.77084712