FastQCFastQC Report
Thu 22 Jan 2015
SV71_ACAGTG_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV71_ACAGTG_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35681283
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC4317071.2098976373691495TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA480100.043.4395641
ATCGGAA483500.043.1434022
CGTCTGA480600.042.67633416
GATCTCG473200.042.6534539
TCTCGTA474350.042.6211941
CTCGTAT474900.042.56235542
ACACGTC483900.042.50082413
CGTATGC477100.042.39438644
TCGGAAG492600.042.314413
ACGTCTG485600.042.31051615
TCGTATG478600.042.3038243
CGGAAGA496850.041.9025654
ATCTCGT486100.041.5909540
CACGTCT497050.041.38547514
GCACACG504050.040.8329311
CACACGT506800.040.62024312
GTATGCC502550.040.2608945
AGTGATC531300.037.7773736
GTGATCT536850.037.4371237
GAACTCC551700.037.166421