FastQCFastQC Report
Thu 22 Jan 2015
SV70_TGACCA_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV70_TGACCA_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42732772
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC5713761.3370908865916773TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA646700.043.8527341
ATCGGAA652700.043.4530642
ACACGTC645850.042.9074713
CGTCTGA646950.042.78002516
TCGGAAG664250.042.6568373
ACGTCTG652300.042.49049415
CGGAAGA669550.042.2452134
TCTCGTA642700.042.16553541
CTCGTAT644450.042.0405642
AATCTCG645100.042.02261439
TCGTATG648800.041.82458543
CACGTCT663550.041.76636514
CGTATGC649000.041.7250344
ATCTCGT655700.041.3570140
GCACACG676350.041.22532711
CACACGT679150.040.98910512
GTATGCC674700.040.1590245
GACCAAT705000.038.1745435
ACCAATC704400.038.16553536
ACTGACC712050.038.00827832
Produced by FastQC (version 0.11.2)