Basic Statistics
Measure | Value |
---|---|
Filename | SV69_CGATGT_L003_R1_001.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 46876120 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC | 419202 | 0.8942762327598787 | TruSeq Adapter, Index 2 (100% over 51bp) |
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA | 56627 | 0.12080138031901957 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 46230 | 0.0 | 43.77923 | 1 |
ATCGGAA | 46975 | 0.0 | 43.10408 | 2 |
ACCGATG | 45835 | 0.0 | 42.59708 | 32 |
CACCGAT | 45890 | 0.0 | 42.555836 | 31 |
CGATGTA | 45755 | 0.0 | 42.543713 | 34 |
CGTCTGA | 46775 | 0.0 | 42.502335 | 16 |
CCGATGT | 45870 | 0.0 | 42.43705 | 33 |
ACACGTC | 47015 | 0.0 | 42.413494 | 13 |
CTCGTAT | 46080 | 0.0 | 42.23389 | 42 |
TCTCGTA | 46170 | 0.0 | 42.185673 | 41 |
ACGTCTG | 47270 | 0.0 | 42.12313 | 15 |
GTCACCG | 46380 | 0.0 | 42.111084 | 29 |
TCGGAAG | 48220 | 0.0 | 41.97716 | 3 |
TCGTATG | 46565 | 0.0 | 41.86164 | 43 |
CGTATGC | 46615 | 0.0 | 41.75882 | 44 |
TATCTCG | 46735 | 0.0 | 41.73344 | 39 |
CGGAAGA | 48570 | 0.0 | 41.58198 | 4 |
TCACCGA | 47350 | 0.0 | 41.167633 | 30 |
CACGTCT | 48690 | 0.0 | 40.93144 | 14 |
ATCTCGT | 47800 | 0.0 | 40.784775 | 40 |