FastQCFastQC Report
Thu 22 Jan 2015
SV69_CGATGT_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSV69_CGATGT_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46876120
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC4192020.8942762327598787TruSeq Adapter, Index 2 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA566270.12080138031901957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA462300.043.779231
ATCGGAA469750.043.104082
ACCGATG458350.042.5970832
CACCGAT458900.042.55583631
CGATGTA457550.042.54371334
CGTCTGA467750.042.50233516
CCGATGT458700.042.4370533
ACACGTC470150.042.41349413
CTCGTAT460800.042.2338942
TCTCGTA461700.042.18567341
ACGTCTG472700.042.1231315
GTCACCG463800.042.11108429
TCGGAAG482200.041.977163
TCGTATG465650.041.8616443
CGTATGC466150.041.7588244
TATCTCG467350.041.7334439
CGGAAGA485700.041.581984
TCACCGA473500.041.16763330
CACGTCT486900.040.9314414
ATCTCGT478000.040.78477540