FastQCFastQC Report
Thu 22 Jan 2015
lane6_Undetermined_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane6_Undetermined_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3813743
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCCGTCCCGATCTCGTAT63730.1671061736462053TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG71050.042.5262411
CACACGT72150.041.6284312
ACACGTC72100.041.62608713
ACGTCTG71700.041.51315715
CACGTCT72800.041.04041714
AGCACAC73900.041.03840610
CGTCTGA72600.040.84519216
GATCGGA76450.040.6453861
AGAGCAC76350.040.163538
CGGAAGA78550.040.0742034
TCGGAAG78750.039.9438363
GAACTCC73650.039.22577321
CTGAACT76100.038.70207219
CTCCAGT70700.038.3786824
CCAGTCA64500.038.29902326
CAGTCAC57000.037.9704127
TGAACTC77700.037.7603320
GAGCACA81650.037.4738049
ATCGGAA84450.037.3016172
ACTCCAG74700.037.07657223
Produced by FastQC (version 0.11.2)