FastQCFastQC Report
Thu 22 Jan 2015
lane5_Undetermined_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane5_Undetermined_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4552623
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC246070.5405015965521415TruSeq Adapter, Index 13 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGCATCTCGTATGCCG77110.1693748856428481TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGCATCTCGTATGCCG75800.16649742357317968TruSeq Adapter, Index 20 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC63100.13860141724891345TruSeq Adapter, Index 8 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC61580.13526268263372568TruSeq Adapter, Index 12 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGGATCTCGTATGCCG51470.11305570437086489TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG303650.043.8698711
ACACGTC302200.043.7751313
CACACGT304350.043.68026412
GATCGGA311100.043.595781
AGCACAC307650.043.50424610
ACGTCTG302400.043.49322515
CACGTCT303550.043.47668514
CGTCTGA302750.043.28325716
CGGAAGA317600.042.969624
TCGGAAG318300.042.9482083
AGAGCAC314550.042.9290128
ATCGGAA319700.042.6545032
GAACTCC305500.042.46236821
GAGCACA317400.042.4513939
CTGAACT309550.042.23392519
TGAACTC309350.042.1448520
GAAGAGC322400.042.0930986
GGAAGAG325250.041.90415
CCAGTCA274200.041.8535626
CTCCAGT290800.041.84956724
Produced by FastQC (version 0.11.2)