FastQCFastQC Report
Thu 22 Jan 2015
SE326_AGTTCC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE326_AGTTCC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6260078
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG2146923.4295419322251255TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC252150.043.31121413
CACACGT252500.043.2868212
GCACACG253000.043.22795511
TCGTATG247350.042.90555245
CACGTCT254550.042.83214614
CGTATCT246800.042.48154439
CTCGTAT249350.042.46216244
TCCGTAT247650.042.44475637
GAACTCC255850.042.33040621
AGCACAC258600.042.32664510
TCTCGTA251000.042.2368143
ACGTCTG259400.042.100715
GTTCCGT249850.041.97196235
CCGTATC249650.041.94250538
TTCCGTA249850.041.8999236
AGAGCAC261800.041.8264738
CGTCTGA260950.041.80919316
GAGCACA262000.041.7859579
GTCACAG254850.041.7576529
TCGGAAG263950.041.6961483
Produced by FastQC (version 0.11.2)