FastQCFastQC Report
Thu 22 Jan 2015
SE325_CGTACG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE325_CGTACG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12367864
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCC1165880.9426688391786974TruSeq Adapter, Index 22 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG137450.041.88775333
CGTACGA137500.041.56162634
CGTATGC142350.039.97172544
GCACACG150850.039.8074911
GTCACCG145900.039.8010229
GTACGAT144300.039.69662535
CACCGTA145650.039.6839731
ACACGTC151950.039.41566513
ACGTCTG151700.039.39163615
TACGATC145400.039.2725236
GTATGCC145350.039.17767345
CACGTCT153000.039.1745814
CTCGTAT145550.039.1083842
CACACGT154150.039.07206712
AGTCACC149150.038.96392428
ACCGTAC149200.038.84530632
CGTCTGA154800.038.5465216
TCGTATG148300.038.51971443
CAGTCAC152150.038.50618727
TCACCGT150000.038.4731430
Produced by FastQC (version 0.11.2)